User: gordo2b

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gordo2b0
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Posts by gordo2b

<prev • 5 results • page 1 of 1 • next >
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FASTQ level UMI correction for TCR analysis
... Hey, I have bulk mouse RNA-seq data from an external lab and am hoping to perform an analysis on the TCR-mapping reads contained within. The library preparation pipeline used resulted in 3 FASTQ files, two 75bp paired read files (R1 and R3), and a UMI file (R2). I was hoping to use MixCR for this, ...
umi tcr fastq mixcr rna-seq written 8 days ago by gordo2b0
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Comment: C: "[E::bgzf_read] Read block operation failed" error - possible truncation/corrupt
... I normally would, hoped not to in this case as this bam is linked to an output that already has already been through a lot of processing. I'll have a think about a way around it. Thanks again for the input! ...
written 17 months ago by gordo2b0
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Comment: C: "[E::bgzf_read] Read block operation failed" error - possible truncation/corrupt
... Added to post, view is also having issues, but not clear why. Thanks for the input btw! ...
written 17 months ago by gordo2b0
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Comment: C: "[E::bgzf_read] Read block operation failed" error - possible truncation/corrupt
... Added to post, looks good ...
written 17 months ago by gordo2b0
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"[E::bgzf_read] Read block operation failed" error - possible truncation/corruption of BAM file
... Hey! I am trying to run velocyto on my BAM file, however I was getting some errors that implied a truncation or corruption of the BAM file. I tested that this wasn't velocyto related by running samtools index on the file, which achieved a highly similar error (below), so it's most likely the file i ...
samtools written 17 months ago by gordo2b0

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