User: leekun4

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leekun40
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Posts by leekun4

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Comment: C: Is it necessary to trim adaptor sequences?
... Thanks for your response. If you don't mind me asking, which software do you use to do your RNA-seq? Thanks for your help. ...
written 8 months ago by leekun40
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Comment: C: Is it necessary to trim adaptor sequences?
... I aligned to a Mus_musculus.GRCm38.98 genome and it's not the same species (I used CD-1). ...
written 8 months ago by leekun40
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Comment: C: Is it necessary to trim adaptor sequences?
... Thanks for sharing the links. These posts help a lot. ...
written 8 months ago by leekun40
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Is it necessary to trim adaptor sequences?
... Hi, I finished mapping with STAR and counting reads using featureCounts. I noticed that I'm getting low alignment reads (~58%, ~20m reads) for each sample. From my understanding, published papers had higher than 80%. According to the FastQC report, per base sequence content did not pass but the pe ...
alignment rna-seq sequencing written 8 months ago by leekun40 • updated 8 months ago by swbarnes27.9k

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