User: Hanna

gravatar for Hanna
Hanna10
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4 months, 2 weeks ago
Joined:
10 months, 1 week ago
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h******@cf.ac.uk

Posts by Hanna

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Comment: C: Associating VDJ clonotyping data with scRNA-seq in Seurat
... I visualise the clonotypes like this (sorry I don't know how to format the R script in a post): for a specific paired alpha and beta TCR or a specific TCRbeta alone with no alpha (replace the asterisks with the exact sequence) E1 <- subset(x = Tcells, subset = cdr3s_aa == "TRA:****************; ...
written 4 months ago by Hanna10
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Comment: C: Associating VDJ clonotyping data with scRNA-seq in Seurat
... Thanks, for some reason I was trying to make subsetting the seurat object far more complicated than it needed to be and your link solved it for me. I wanted to subset on the top 10 from each group, so this was easy to do as the names have remained the same, but now I can see that if I do want to loo ...
written 10 months ago by Hanna10
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Comment: C: Associating VDJ clonotyping data with scRNA-seq in Seurat
... Thanks for this really helpful tutorial. I have now added my VDJ data to two seurat objects ("before" and "after" treatment) and then intergrated them. I now have two questions that I was wondering if you could help me with please? I would like to subset my Seurat object so that I retain the top 10 ...
written 10 months ago by Hanna10

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