User: mejait99

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mejait9920
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Morocco
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9 hours ago
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4 months, 1 week ago
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Posts by mejait99

<prev • 36 results • page 1 of 4 • next >
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Comment: C: assembly of fastq_pair results (4 files )
... Thank you very much for your message , please i extracted unmapped reads frow data and i don't exactly in which step i had the error first i mapped my reads on the eference using bowtie2 after that i transformed sam file to bam file using samtools and i extracted unmapped reads using samtools v ...
written 11 hours ago by mejait9920
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assembly of fastq_pair results (4 files )
... Hello everyone I have two files containing reads that I want to assembly I run spades and it shows this error message Error log: == Running assembler: K27 0:00:00.000 4M / 4M INFO General (main.cpp : 74) Loaded config from /data/friesen/testdrive/agro-dir/spades_as ...
software error assembly alignment sequence written 12 hours ago by mejait9920 • updated 11 hours ago by RamRS26k
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Comment: C: How to obtain my forward and reverse reads from sam / bam file
... i did the same thing on other reads that i download and shovill works well , anyway i will try to download pilon changes and see , thatnk you ahh i just clarified the situation , thank you ...
written 6 days ago by mejait9920
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Comment: C: How to obtain my forward and reverse reads from sam / bam file
... hello . because the other solutions in the previous publication didn't work for me , so i tried in this question to explain more the situation and to explain the solution that didn't work for me , so it's technically not the same ...
written 6 days ago by mejait9920
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How to obtain my forward and reverse reads from sam / bam file
... Hello everyone I hope you and your family and friends doing well please i need help in something my goal was to obtain unmapped reads i have my forward and reverse reads , first i mapped my data to reference genome using bowtie2 i obtained sam file and i ransfered it to bam file using samtools ...
software error assembly sequence written 6 days ago by mejait9920
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Comment: C: how to split forward and reverse reads from sam file
... Okay , thank you very much ...
written 7 days ago by mejait9920
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Comment: C: how to split forward and reverse reads from sam file
... Thank you very much for your answer i did this command to obtain unmapped reads samtools view -h -f 4 nom_de_souche_Resultat.bam > nom_de_souche_Unmapped_reads.bam i save all the files so i can fix the error in any step i mapped my reads against the eference using bowtie2 after that i ran ...
written 7 days ago by mejait9920 • updated 7 days ago by lieven.sterck7.2k
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how to split forward and reverse reads from sam file
... Hello everyone, I have question please I have bam file containing unmapped reads that I want to assemble. I tried samtools fastq to obtain the two files forward and reverse I did this command samtools fastq -1 wPru_1_Unmapped_reads.fastq -2 wPru_2_Unmapped_reads.fastq wPru_Unmapped_reads ...
assembly alignment sequencing written 7 days ago by mejait9920 • updated 7 days ago by genomax80k
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Fastqc shows error message
... Hello everyone i hope you and your family and friends are doing fine please i have question i m remotely connected to the lab server , i want to check the quality of the my reads and i want to use fastqc for that . normaly it works well on the lab computer but what i tried to run on my comput ...
genome assembly next-gen sequencing written 7 days ago by mejait9920 • updated 7 days ago by ATpoint31k
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Comment: C: The best k mer to use for illumina data
... Thank you very much for your answer , I'm using shovill for the assembly please tel me what things i should take in consideration to choose the best k-mer ( i just know , the number of contigs and N50 ) are they enough ...
written 8 days ago by mejait9920

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