User: michelle.piquet

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Posts by michelle.piquet

<prev • 25 results • page 1 of 3 • next >
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Adding certain barcodes to metadata
... I want to upload an excel file sheet that has certain barcodes that I would like to show on my umap. How do I go about adding the file and linking it to the metadata? Below is my following code. # set up the working directory wd = "/home/PTX_AAC656 setwd(wd) # load counts ...
seurat scrnaseq written 5 months ago by michelle.piquet50
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Comment: C: differential expression volcano plot
... Hi Kevin, I don't understand what I'm suppose to do. How do I go about binding everything in the file? I tried to change the names in the file to just log2foldchange and pvalue but when I upload the dataset, a 1-20 is added to the end of each. ...
written 9 months ago by michelle.piquet50
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Comment: C: differential expression volcano plot
... I already did differential expression for both conditions, I just need to to plot them into a volcano plot. ...
written 9 months ago by michelle.piquet50
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Comment: C: differential expression with Seurat
... No, just between the treated and untreated conditions. I already have the differential expression analysis excel file, I'm just stuck on how to merge everything together. I know how to do a volcano plot for each cluster and then merge but can't seem to just merge everything into one and do the volca ...
written 9 months ago by michelle.piquet50
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Comment: C: differential expression with Seurat
... It's both. I used Seurat to generate the differential expression file and now don't know how to combine all the information from the file into a volcanoplot within Seurat or with EnhancedVolcano, don't know which would allow me to merge all the information together. I did the merge for 2 out of th ...
written 9 months ago by michelle.piquet50
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Comment: C: differential expression with Seurat
... Yes. I have the csv file with the differential expression for both treated and untreated conditions. There are 20 total clusters, how do I go about merging the results table since all the p values and log fold change values are already in my file. They're just labeled based on which cluster it belon ...
written 9 months ago by michelle.piquet50 • updated 9 months ago by RamRS30k
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Comment: C: differential expression with Seurat
... I have just loaded each count file into Seurat. Now, I'm trying to combine all treated and untreated samples and do differential expression and then do a volcano plot at the end. ...
written 9 months ago by michelle.piquet50
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differential expression volcano plot
... Hello, I have generated the counts from 10x Chromium and then generated differential expression for two conditions, untreated and treated. I have done volcano plots for each cluster and then combined them all into one. Is there a way to just do a volcano plot from the excel file all at once, rathe ...
R rna-seq sequencing written 9 months ago by michelle.piquet50
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Comment: C: Volcano plot for multiple clusters
... These are the headers. Feature ID Feature Name Cluster 1 Mean Counts Cluster 1 Log2 fold change Cluster 1 Adjusted p value Cluster 2 Mean Counts Cluster 2 Log2 fold change Cluster 2 Adjusted p value Cluster 3 Mean Counts Cluster 3 Log2 fold change Cluster 3 Adjusted p value Cluster 4 Mean Coun ...
written 9 months ago by michelle.piquet50 • updated 9 months ago by Kevin Blighe65k
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Comment: C: Volcano plot for multiple clusters
... How do I go about merging the results table since all the p values and log fold change values are already in my file. They're just labeled based on which cluster it belongs to? Thanks again for your help. ...
written 9 months ago by michelle.piquet50

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