Moderator: h.mon

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h.mon24k
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Posts by h.mon

<prev • 3,503 results • page 1 of 351 • next >
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Comment: C: Fastq Convert To Fasta
... Very late indeed, as there already is an almost identical answer, posted years ago (here is the [direct link][1] to the answer). The difference being your answer has `sed 's/^@/>/'`, while the posted has `sed 's/^@/>/g'`. [1]: https://www.biostars.org/p/85929/#85955 ...
written 2 days ago by h.mon24k
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Comment: C: Which of the following four genome coordinates is correct for the first coding e
... This seems like homework, if it is, please search the information yourself, it is probably against the code of conduct to ask someone else to do your homework. There are several mouse genome versions (e.g. mm9 mm10), several annotations (e.g., Ensembl, NCBI, ENCODE), and several isoforms of the gen ...
written 2 days ago by h.mon24k
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Comment: C: getting the test results of multiple transcripts; even though, I am using gene
... Please edit your post to include the commands you used - we have no idea of what you did, parameters chosen, and so forth. ...
written 2 days ago by h.mon24k
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Comment: C: Phyloseq to DESeq2 for 16s amplicon sequencing data
... Could you please format your code? After highlighting the text you want to format, use the icon below: ![ScreenCap][1] [1]: http://i66.tinypic.com/33kdnjt.png ...
written 2 days ago by h.mon24k
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Comment: C: Is there a way to run RAST annotation locally?
... RAST I don't know, but you can install and run the [NCBI Prokaryotic Genome Annotation Pipeline][1]. [1]: https://github.com/ncbi/pgap ...
written 2 days ago by h.mon24k
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Comment: C: Error in differential analysis for samples with different time points
... The above has the very same problem as your original post. You need more replicates **per treatment** - each day has only one sample, you need more per same day. ...
written 3 days ago by h.mon24k
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Comment: C: basic experimental methods book for bioinformaticians
... Well then, by your definition, whatever you do won't help to change their minds. Besides, this terminology is not helpful, on the contrary, is alienating. You are just making your task harder if you start by calling derogatory names the people you want to convince. Sorry, didn't see the question an ...
written 3 days ago by h.mon24k
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Answer: A: Error in differential analysis for samples with different time points
... The design matrix is not full rank because you have only one sample (no biological replicates) per combination of treatment. You may either drop day from the analysis, or add more samples per treatment. ...
written 3 days ago by h.mon24k
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Comment: C: basic experimental methods book for bioinformaticians
... >sentimentalists What are sentimentalists? ...
written 3 days ago by h.mon24k
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Comment: C: (edgeR-related) DGElist error message 'Negative counts not allowed'.
... Take everything I say bellow with a grain of salt, as I always performed summarization over genes, never over exons, and I don't know the recommended settings for exon-level summarization. I suppose there are non-unique Geneids because you are summarizing over exons (flag `-f`) instead of over gen ...
written 4 days ago by h.mon24k

Latest awards to h.mon

Good Answer 1 hour ago, created an answer that was upvoted at least 5 times. For A: convert STAR ReadsPerGene.out.tab to Deseq2 count table
Teacher 9 hours ago, created an answer with at least 3 up-votes. For A: RNA-seq: Can we obtain distances between samples of different experiments?
Scholar 9 hours ago, created an answer that has been accepted. For A: Find bams with identical names and merge them
Commentator 21 hours ago, created a comment with at least 3 up-votes. For C: STAR align multiple files
Appreciated 4 days ago, created a post with more than 5 votes. For Where did EMBOSS go?
Scholar 5 days ago, created an answer that has been accepted. For A: Find bams with identical names and merge them
Teacher 5 days ago, created an answer with at least 3 up-votes. For A: RNA-seq: Can we obtain distances between samples of different experiments?
Teacher 5 days ago, created an answer with at least 3 up-votes. For A: RNA-seq: Can we obtain distances between samples of different experiments?
Scholar 5 days ago, created an answer that has been accepted. For A: Find bams with identical names and merge them
Teacher 5 days ago, created an answer with at least 3 up-votes. For A: RNA-seq: Can we obtain distances between samples of different experiments?
Scholar 5 days ago, created an answer that has been accepted. For A: Find bams with identical names and merge them
Teacher 6 days ago, created an answer with at least 3 up-votes. For A: RNA-seq: Can we obtain distances between samples of different experiments?
Appreciated 12 days ago, created a post with more than 5 votes. For Benchmarking bioinformatics tools
Teacher 13 days ago, created an answer with at least 3 up-votes. For A: Are secondary alignments used by any tool?
Scholar 13 days ago, created an answer that has been accepted. For A: Find bams with identical names and merge them
Scholar 19 days ago, created an answer that has been accepted. For A: Find bams with identical names and merge them
Scholar 24 days ago, created an answer that has been accepted. For A: Find bams with identical names and merge them
Teacher 24 days ago, created an answer with at least 3 up-votes. For C: Identify insertions with IGV
Teacher 24 days ago, created an answer with at least 3 up-votes. For A: Are secondary alignments used by any tool?
Teacher 24 days ago, created an answer with at least 3 up-votes. For A: Are secondary alignments used by any tool?
Scholar 24 days ago, created an answer that has been accepted. For A: Find bams with identical names and merge them
Scholar 25 days ago, created an answer that has been accepted. For A: Find bams with identical names and merge them
Scholar 27 days ago, created an answer that has been accepted. For A: Find bams with identical names and merge them
Teacher 4 weeks ago, created an answer with at least 3 up-votes. For A: Are secondary alignments used by any tool?
Teacher 4 weeks ago, created an answer with at least 3 up-votes. For A: Are secondary alignments used by any tool?

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