User: robert.yzc

gravatar for robert.yzc
robert.yzc0
Reputation:
0
Status:
New User
Last seen:
3 months, 2 weeks ago
Joined:
3 months, 3 weeks ago
Email:
r*********@gmail.com

Profile information, website and location are not shown for new users.

This helps us discourage the inappropriate use of our site.

Posts by robert.yzc

<prev • 6 results • page 1 of 1 • next >
0
votes
1
answer
331
views
1
answers
Comment: C: Error when running cellranger mkref single nuclei RNA-Seq
... Ah I see, thanks! Just accepted it. ...
written 3 months ago by robert.yzc0
0
votes
1
answer
331
views
1
answers
Comment: C: Error when running cellranger mkref single nuclei RNA-Seq
... Hey genomax! I added additional memory and haven't errored out after ~25 minutes... Looking good so far! I can't believe the fix was (potentially) this simple. Thanks for riding this journey with me..! ...
written 3 months ago by robert.yzc0
0
votes
1
answer
331
views
1
answers
Comment: C: Error when running cellranger mkref single nuclei RNA-Seq
... An update: So far, I haven't errored out after adding the additional memory buffer!! Fingers crossed, but it looks like that fixed it. Thanks for your help, you won't believe how long I spent trying to troubleshoot this... Any way I can "accept" a formal answer like stackexchange? I'm new to biosta ...
written 3 months ago by robert.yzc0
0
votes
1
answer
331
views
1
answers
Comment: C: Error when running cellranger mkref single nuclei RNA-Seq
... Thanks for testing this out genomax! As swbarnes2 pointed out, I need to make a custom reference to only include "transcripts" converted to "exons" so that introns are retained in the .gtf file. It's reassuring to see that on 32G, creating the reference works. I'll report back as soon as I get acces ...
written 3 months ago by robert.yzc0
0
votes
1
answer
331
views
1
answers
Comment: C: Error when running cellranger mkref single nuclei RNA-Seq
... I hope it is such a simple solution! Thanks for taking the time to respond so quickly. I will request the cluster to provide me additional memory (100 GB) and see if the error persists. I'll report back on the results, regardless of what they are. ...
written 3 months ago by robert.yzc0
2
votes
1
answer
331
views
1
answer
Error when running cellranger mkref single nuclei RNA-Seq
... Hi everyone - I'm trying to make a custom reference for a 10x Genomics v3 single-nuclei RNA-Seq run. According to the instructions on 10x's website (https://support.10xgenomics.com/single-cell-gene-expression/software/pipelines/latest/advanced/references) I can use the following commands: # ...
10x single-cell rna-seq rna-seq written 3 months ago by robert.yzc0 • updated 3 months ago by genomax80k

Latest awards to robert.yzc

No awards yet. Soon to come :-)

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 2152 users visited in the last hour