User: wu.zhiqiang.1020

Reputation:
20
Status:
New User
Location:
United States
Last seen:
1 week, 5 days ago
Joined:
5 years ago
Email:
w***************@gmail.com

about me

Posts by wu.zhiqiang.1020

<prev • 36 results • page 1 of 4 • next >
0
votes
1
answer
328
views
1
answers
Comment: C: how to extract the longest isoform from trinity outfils
... thanks, I find it and make it go through. thanks again ...
written 8 months ago by wu.zhiqiang.102020
0
votes
1
answer
328
views
1
answers
Comment: C: how to extract the longest isoform from trinity outfils
... It need other pm file to load and the same time. I find this link. But I have to search other more pm files also thanks ...
written 8 months ago by wu.zhiqiang.102020
0
votes
1
answer
328
views
1
answer
how to extract the longest isoform from trinity outfils
... Dear all, I want to filtered the Trinity outfile for RNAseq assemble. I know this is not the best choice, and there are some scripts online. but they are all not good to use in my data, Could you give me some suggestion on this? my data like this: (Start with > for name) >TRINITY_DN66086_c ...
assembly rna-seq written 8 months ago by wu.zhiqiang.102020 • updated 8 months ago by h.mon9.2k
0
votes
2
answers
513
views
2
answers
Comment: C: how to use CD_HIT to remove the redundant sequence from trinity output file
... Hi st.ph.n I tried your script, it shown error as Traceback (most recent call last): File "get_longest_iso.py", line 8, in if line.startswith(">"): NameError: name 'line' is not defined could you help me to fix this? I do not know the python. thanks My trinity out start as: >TRI ...
written 8 months ago by wu.zhiqiang.102020
0
votes
2
answers
513
views
2
answers
Comment: C: how to use CD_HIT to remove the redundant sequence from trinity output file
... thanks。 I need to read Trinity again. ...
written 8 months ago by wu.zhiqiang.102020
0
votes
2
answers
513
views
2
answers
Comment: C: how to use CD_HIT to remove the redundant sequence from trinity output file
... it would be helpful to keep the longest isoforms. but I still could not remove the duplicates from other contrig. right? thanks ...
written 8 months ago by wu.zhiqiang.102020
0
votes
2
answers
513
views
2
answers
Comment: C: how to use CD_HIT to remove the redundant sequence from trinity output file
... yes, I just want to have one longest isoform. How can I realize that? ZQ ...
written 8 months ago by wu.zhiqiang.102020
0
votes
2
answers
513
views
2
answers
Comment: C: how to use CD_HIT to remove the redundant sequence from trinity output file
... you mean -c and -t right? ...
written 8 months ago by wu.zhiqiang.102020
0
votes
2
answers
513
views
2
answers
how to use CD_HIT to remove the redundant sequence from trinity output file
... Dear all, I am trying to use CD-hit to remove the duplicates from the file that is the output from trinity (RNA seq assembly). I used the following parameters: cd-hit-est -i in.fasta -o out_cdhit90.fasta -c 0.90 -n 9 -d 0 -M 0 -T 0 But the output file still contains lots of small or fragmented s ...
assembly rna-seq written 8 months ago by wu.zhiqiang.102020 • updated 8 months ago by st.ph.n1.9k
0
votes
0
answers
313
views
0
answers
how to use bbnorm to subset the data
... I have a pooled RNA seq data from 5 to 10 individuals at different stages. I want to assemble them and combine all of data as one big fastq files. I use the bbnorm to reduce the replicates. But I have a question about how to set the parameters as: target=100 (tgt) Target normalization ...
assembly bbmap bbnorm rna-seq written 9 months ago by wu.zhiqiang.102020 • updated 9 months ago by genomax37k

Latest awards to wu.zhiqiang.1020

Popular Question 7 months ago, created a question with more than 1,000 views. For Error in vcf, samtools
Popular Question 11 months ago, created a question with more than 1,000 views. For Error in vcf, samtools
Popular Question 2.5 years ago, created a question with more than 1,000 views. For How To Delete Some Rgs From One Bam File
Popular Question 2.5 years ago, created a question with more than 1,000 views. For Error in SNP calling using samtools and bcftools

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 1412 users visited in the last hour