User: manjovial

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manjovial0
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Posts by manjovial

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Can loess.normalization be used for normalizing RNASeq performed with two ERCC mixes?
... Hi, I am working on RNAseq data where my colleague has used two ERCC mixes , Mix 1 for tests and Mix 2 for WT. https://www.thermofisher.com/order/catalog/product/4456740 So, I have 92 ERCC transcripts with different expected fold change. I am not sure how to provide the information for two mixes wh ...
ercc normalization rna-seq written 2.6 years ago by manjovial0 • updated 19 months ago by Kevin Blighe45k
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Comment: C: Tissue-Specific And Housekeeping Gene Ratio
... False negative here means genes which are really housekeeping (by prior knowledge or swissprot) are not showing signal intensities above decided cut-off value in all the tissues . ...
written 6.6 years ago by manjovial0
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Comment: C: Tissue-Specific And Housekeeping Gene Ratio
... Its human's gene expression data. I am looking for absolute present and absent (which is decided by taking a cut-off value) in both the cases. I am studying certain properties of tissue-specific genes and housekeeping genes and want to show that my results are gene expression data independent. ...
written 6.6 years ago by manjovial0
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Tissue-Specific And Housekeeping Gene Ratio
... I work both on RNAseq data ( 10 tissues) and Microarray data ( 70 tissues) . The problem is that I find microarray data to be false negative for many of the housekeeping genes. The ratio of tissue-specific to housekeeping genes from RNAseq data comes around 1:10 whereas by microarray it comes 1:2 . ...
gene microarray rna-seq written 6.6 years ago by manjovial0 • updated 6.6 years ago by Michael Dondrup46k

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Popular Question 6.6 years ago, created a question with more than 1,000 views. For Tissue-Specific And Housekeeping Gene Ratio

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