User: Rahul Tyagi

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Posts by Rahul Tyagi

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mitochondria in single nuclear RNAseq
... for snRNAseq, do people use mitochondrial filtering? I'm not sure if this still makes sense even though we are working with data from nuclei rather than whole cells, but I do see some expression of mito genes in my data. Also, is it preferable to filter the cells based on mito content or to regres ...
snrnaseq single cell rnaseq scrnaseq seurat written 6 weeks ago by Rahul Tyagi0
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issues with single nuclei sequencing
... Hi All, I am working on a set of single-nuclei sequencing samples. Does someone know why I might be getting a bimodal distribution in one of my samples (both in features/cell and reads/cell)? The modes are very different (1000 genes vs 150 genes). The other samples almost exclusively contain the "s ...
scseq single nuclei sequencing rna-seq seurat written 7 months ago by Rahul Tyagi0 • updated 6 months ago by Biostar ♦♦ 20

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