User: skymningen

gravatar for skymningen
skymningen330
Reputation:
330
Status:
Trusted
Location:
Germany
Last seen:
3 years, 9 months ago
Joined:
6 years, 3 months ago
Email:
b*********@googlemail.com

passionate professional bioinformatician and hobby molecular biologist

Posts by skymningen

<prev • 44 results • page 1 of 5 • next >
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Comment: C: ERROR while using PBcR
... I had similar problems and went through a lot of searching, reinstalling AMOS and such without being able to solve it. In the end I decided to use falcon_sense by adding the  -pbCNS flag and figured it was maybe some problem specific to server configuration. Now I think this might really be a bug in ...
written 4.9 years ago by skymningen330
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Comment: C: Perl Program Of Genes
... Unfortunately this is a statement, not a question. Please state, what you have already tried and what is your question. ...
written 5.2 years ago by skymningen330
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Comment: C: Installed Bwa But There Are No Bwa Files Produced In Galaxy-Dist Folder
... You are supposed to make the .loc files yourself, so they match the sample, but include the right paths to were your BWA indices are stored. The second sentence of the header for the example file is "You will need to create these data files and then create a bwa_index.loc file similar to this one (s ...
written 5.2 years ago by skymningen330
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Comment: C: Partial Codon Or Missing Stop Codon
... I just looked at the first one. It is annotated as a disrupted gene, so possibly disrupted by some in vitro modification. ...
written 5.3 years ago by skymningen330
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Comment: C: What Would Be The Next Analysis Steps After Producing Alignments With Blastx
... The further analysis completely depends on what information you wanted to get out of what kind of sequence data. Is this data from one genome, an environmental sample, are those raw reads, assembled sequences, gene sequences? ...
written 5.3 years ago by skymningen330
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Comment: C: A Universal/Common Biorep System
... You would still recognise the well known, continuous posters from their writing style and the kind of topic they replied to. But I like the idea to at least add measures like Istvan suggested to the already available rep gaining (and loosing). Which is the main advantage of reputation over starbucks ...
written 5.5 years ago by skymningen330
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Answer: A: Near Target Reads In Whole Exome Data
... That depends on the target capture method. For example, for Agilent SureSelect, you can capture up to 200 bp next to the target (at least they state that, I haven't had the chance to check yet). So, preferably to determine the amount of near-target region that you need to consider, best check the sp ...
written 5.5 years ago by skymningen330
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Comment: C: How To Remove Common Variants Present In Two Vcf Files?
... You should at least add, that (as far as I understand from your website) this only works for human data. ...
written 5.7 years ago by skymningen330
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Comment: C: Extracting Read Pairs From Region So That All Pairs Remain Intact
... Try: bamtools filter -isMateMapped true -in myBAM.4genes.bam -out myBAM.4genes.bothmapped.bam ...
written 5.7 years ago by skymningen330
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Comment: C: Query Regarding Bacterial Strain
... Why don't you just write the corresponding author to ask it? ...
written 5.7 years ago by skymningen330

Latest awards to skymningen

Popular Question 4.7 years ago, created a question with more than 1,000 views. For Bcftools Back And Forth Conversion Error
Supporter 5.1 years ago, voted at least 25 times.
Teacher 5.8 years ago, created an answer with at least 3 up-votes. For A: Isn'T A Prokaryotic Gene Continuous..?
Teacher 5.8 years ago, created an answer with at least 3 up-votes. For A: Database Of Cultured Bacterial 16S Rdna Sequences
Good Answer 6.0 years ago, created an answer that was upvoted at least 5 times. For A: Are We Rude/Do We Expect Too Much From People Asking Questions On This Forum?
Teacher 6.0 years ago, created an answer with at least 3 up-votes. For A: Are We Rude/Do We Expect Too Much From People Asking Questions On This Forum?
Teacher 6.3 years ago, created an answer with at least 3 up-votes. For A: A Farewell To Bioinformatics

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