User: moranr

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moranr240
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Posts by moranr

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Comment: C: How can I demultiplex two Library preps(Single Index and Dual Index) using bcl2f
... If we had something like this : IsIndexedRead=N Y N NumCycles=151 8 151 Number=1 2 3 This indicates that the RunInfo.xml was correct ?? Thanks, ...
written 6 months ago by moranr240
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Comment: C: How can I demultiplex two Library preps(Single Index and Dual Index) using bcl2f
... Thanks for the clarification. ...
written 6 months ago by moranr240
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Comment: C: How can I demultiplex two Library preps(Single Index and Dual Index) using bcl2f
... Could it be that this was the XML for one of the library preps and not both (i.e. the single index prep)? ...
written 6 months ago by moranr240
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Comment: C: How can I demultiplex two Library preps(Single Index and Dual Index) using bcl2f
... As far as I am aware, yes ? . Should it look something like this : ? ...
written 6 months ago by moranr240
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Comment: C: How to demultiplex dual-index NextSeq data for random index 2?
... Thanks, Created a question :https://www.biostars.org/p/298822/ ...
written 6 months ago by moranr240
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How can I demultiplex two Library preps(Single Index and Dual Index) using bcl2fastq.
... How can I demultiplex two Library preps(Single Index and Dual Index) ? 4 samples were used to build two different libraries Dual Index and Single Index. We mixed both libraries and added all the 8 samples to both lanes of an S2 flowcell. Now we have the BCL files from this run and the problem we ...
fastq sequencing bcl written 6 months ago by moranr240 • updated 6 months ago by genomax54k
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Comment: C: How to demultiplex dual-index NextSeq data for random index 2?
... Thanks Genomax , i tried both post's suggestions and still not working for me. I'll explore more thanks. ...
written 6 months ago by moranr240
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Comment: C: How to demultiplex dual-index NextSeq data for random index 2?
... Index_2 has 4 unique indexes as 4 samples are Dual index and 4 have a single index. ...
written 6 months ago by moranr240
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Comment: C: How to demultiplex dual-index NextSeq data for random index 2?
... Did you ever find a way to do this ? ...
written 6 months ago by moranr240
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Answer: A: comparing protein sequences to identify conserved regions
... BLAST will do what you want depending on your resources. You want to run an all vs all protein BLAST. Depending on the number of seqs you have, you may need to run on a computing cluster of some kind to speed it up and run across multiple cores(which I think you definitely will with so many str ...
written 16 months ago by moranr240

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