User: priscilladraj

gravatar for priscilladraj
Reputation:
10
Status:
New User
Location:
Last seen:
1 day, 1 hour ago
Joined:
1 month ago
Email:
p************@gmail.com

Posts by priscilladraj

<prev • 10 results • page 1 of 1 • next >
0
votes
0
answers
72
views
0
answers
Bowtie2 or Bowtie
... Hi, I have demultiplexed reads for each of my library. So each libary contains read 1 and read 1 as I used dual indexex. Hence, I combined both reads (read 1 and read 1 specific for each pool) into one fastq.gz file. As for my reference file, I indexed the fasta file containning all my refere ...
alignment bowtie written 7 days ago by priscilladraj10 • updated 7 days ago by lieven.sterck8.5k
1
vote
2
answers
143
views
2
answers
Answer: A: filter illumina index
... thanks I manages to resolve it demultiplexing it again bcl base caller software and it worked this time because I learned that teh Iseq reads i5 from reverse complement direction. So its my mistake of not knowing this. ...
written 7 days ago by priscilladraj10
0
votes
0
answers
45
views
0
answers
Comment: C: convert multiple sequences in excel to fasta file
... yes, it did help. thanks so much. I am not sure why I count not find this earlier. thanks again :) ...
written 9 days ago by priscilladraj10
1
vote
0
answers
45
views
0
answers
(Closed) convert multiple sequences in excel to fasta file
... Hi, I have a CSV file with just two columns(ref ID and ref sequence). I would like convert this to a fasta file format so that I can align my fastq file with these reference sequences. How do I do the conversion from csv to fasta? Kindly let me know please. Thanks Pris ...
csv convert to fasta written 9 days ago by priscilladraj10
1
vote
2
answers
143
views
2
answers
filter illumina index
... Hi, I ran an iseq illumina sequencing on my libary. And due some sample prepration problem, I have a low concentration of my libary. Hence, after sequecing and base calling, the iseq base caller grouped all my reads (dual index, 3 pooled libary = 6 reads total) under a undetermined folder. I want ...
0
votes
1
answer
76
views
1
answers
Comment: C: Parse error at line.....
... Hey thanks genomax . I am still new so this small information is also useful for me :D ...
written 12 days ago by priscilladraj10
0
votes
1
answer
76
views
1
answers
Comment: C: Parse error at line.....
... hey thanks it worked :D ...
written 13 days ago by priscilladraj10
3
votes
1
answer
76
views
1
answer
Parse error at line.....
... Hi I am trying to convert sam file to bam file and encountered this problem . Not sure how to troubleshoot, Can someone help please as I am very new to bioinformatics samtools view -S -b aln2.sam > aln2.bam [W::sam_read1] Parse error at line 753646 [main_samview] truncated file. ...
alignment written 13 days ago by priscilladraj10 • updated 13 days ago by lieven.sterck8.5k
0
votes
1
answer
125
views
1
answers
Answer: A: analyse bam file
... Thanks I actually used samstat I was able to get the number aligned reads. I am new to Biostar too , so i didnt see this replies until today. Thanks :) ...
written 29 days ago by priscilladraj10
0
votes
1
answer
125
views
1
answer
analyse bam file
... Hi, I am really new to any bioinformatics analysis. So please, if you could answer me in the most simplest form I would trully appreciate it. I have two bam files (bam 1 and bam 2) that were generated when I aligned one fastq read file to two reference sequences (ref 1 ref2) fasta format. When I v ...
alignment sequencing written 4 weeks ago by priscilladraj10 • updated 29 days ago by wljlinksly50

Latest awards to priscilladraj

Scholar 7 days ago, created an answer that has been accepted. For A: filter illumina index

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 1138 users visited in the last hour