User: DeadlyFall

gravatar for DeadlyFall
DeadlyFall0
Reputation:
0
Status:
New User
Last seen:
3 days, 17 hours ago
Joined:
2 weeks, 6 days ago
Email:
g************@cuanschutz.edu

Profile information, website and location are not shown for new users.

This helps us discourage the inappropriate use of our site.

Posts by DeadlyFall

<prev • 5 results • page 1 of 1 • next >
0
votes
0
answers
309
views
0
answers
Comment: C: DESeq2: When to split multiple group samples for 'more' accurate sizefactors, di
... Just wanted to bump this as I am having the same problem. In my experiment, samples were taken from patients and cultured, then exposed to an immune stimulating compound. I've been asked to make sure the cell lines match the original patient sample and then to look at the effect of the added compoun ...
written 4 days ago by DeadlyFall0
0
votes
1
answer
409
views
1
answers
Comment: C: Best package to cluster a single cell dataset using marker gene lists retrieved
... I'll add my recommendation for this package. I've used it on several different projects using Seurat objects. Very simple to use and does a great job, especially with immune cells. Thank you for helping to make this package! ...
written 18 days ago by DeadlyFall0
0
votes
0
answers
97
views
0
answers
Comment: C: How to handle multivariable experiment with potential confounding and no biologi
... Thank you for the input! I have performed this using the top 25 genes from one of the comparisons and luckily the cell lines did not make too much of a difference . Most of the variation was explained by PC1 and there was a nice split between disease within a condition, with cell lines scattering a ...
written 20 days ago by DeadlyFall0
0
votes
0
answers
97
views
0
answers
Comment: C: How to handle multivariable experiment with potential confounding and no biologi
... Thank you for confirming. It is common that I receive such experiments unfortunately and it is not fun to tell the researcher that their data won't allow them to answer their question : / It's nice to have others state the same so they know it is not just my opinion. ...
written 20 days ago by DeadlyFall0
2
votes
0
answers
97
views
0
answers
How to handle multivariable experiment with potential confounding and no biological replicates in DESeq2
... Hello all, I'm currently attempting to analyze some RNA-Seq data using DESeq2 and having a hard time since this experiment does not have biological replicates, yet it has 3 different variables. I question whether I should even be using DESeq2 in this situation and what a better alternative would be ...
R deseq2 rna-seq written 20 days ago by DeadlyFall0

Latest awards to DeadlyFall

No awards yet. Soon to come :-)

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 1972 users visited in the last hour