User: MEITUO
MEITUO • 10
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Posts by MEITUO
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... thanks! and is the bam file that the qc reads mapping back to assembly contig? do this need filter low mapped reads or just can use it directly after bwa or bowite?? ...
written 7 weeks ago by
MEITUO • 10
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... metagenic data, the contigs after assembly ...
written 7 weeks ago by
MEITUO • 10
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... HI everyone!
i am trying to calculate the gene distance of assembly contigs by closest-features. but this tool requires a bed format input file. how can i get this bed-format file? ...
written 7 weeks ago by
MEITUO • 10
• updated
7 weeks ago by
jordi.planells • 330
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Comment:
C: spades assembly sample
... hi!
thanks for your answer.
i had rerun this, but still same error ...
written 9 weeks ago by
MEITUO • 10
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... hi everyone!
i am using spades to assembly my sample. the command line:
spades.py --meta -1 qc_reads/DP_Sample016_S16_adapter_phix_moving_sickle_R1_sss.fastq.gz -2 qc_reads/DP_Sample016_S16_adapter_phix_moving_sickle_R2_sss.fastq.gz -m 500 -t 35 -k 21,33,55 -o assembly_plasmidome/DP_Sample016_ ...
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... hi everyone!
i am using blast+ to make db, but i one error
command line:
ncbi-blast-2.10.1+/bin/makeblastdb -in plasmid_refseq.fasta -dbtype nucl -parse_s
error:
Keep MBits: T
Maximum file size: 1000000000B
No volumes were created.
Error: mdb_env_open: Cannot allocate m ...
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... thnaks, i will try to look at it ...
written 3 months ago by
MEITUO • 10
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... i had used randomreads.sh, but it is quite slow if i want to get 10,000,000 reads, so i try to use other tools, whether these are more quickly ...
written 3 months ago by
MEITUO • 10
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... ART/art_bin_MountRainier/art_illumina -ss HS25 -sam -i precision_dataset.fna -l 150 -f 1 -o art-paired-dat
====================ART====================
ART_Illumina (2008-2016)
Q Version 2.5.8 (June 7, 2016)
Contact: Weichun Hu ...
written 3 months ago by
MEITUO • 10
• updated
3 months ago by
Mensur Dlakic • 8.1k
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... hi!
the input file of DAVID is a gene list, is the gene list in the emapper.annotations file? or how can i get the gene list? ...
written 3 months ago by
MEITUO • 10
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