User: MEITUO

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MEITUO10
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Posts by MEITUO

<prev • 14 results • page 1 of 2 • next >
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Comment: C: how to get input file of closest-features a bed-format file
... thanks! and is the bam file that the qc reads mapping back to assembly contig? do this need filter low mapped reads or just can use it directly after bwa or bowite?? ...
written 7 weeks ago by MEITUO10
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Comment: C: how to get input file of closest-features a bed-format file
... metagenic data, the contigs after assembly ...
written 7 weeks ago by MEITUO10
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how to get input file of closest-features a bed-format file
... HI everyone! i am trying to calculate the gene distance of assembly contigs by closest-features. but this tool requires a bed format input file. how can i get this bed-format file? ...
genome gene sequencing written 7 weeks ago by MEITUO10 • updated 7 weeks ago by jordi.planells330
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Comment: C: spades assembly sample
... hi! thanks for your answer. i had rerun this, but still same error ...
written 9 weeks ago by MEITUO10
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spades assembly sample
... hi everyone! i am using spades to assembly my sample. the command line: spades.py --meta -1 qc_reads/DP_Sample016_S16_adapter_phix_moving_sickle_R1_sss.fastq.gz -2 qc_reads/DP_Sample016_S16_adapter_phix_moving_sickle_R2_sss.fastq.gz -m 500 -t 35 -k 21,33,55 -o assembly_plasmidome/DP_Sample016_ ...
assembly tool written 9 weeks ago by MEITUO10 • updated 9 weeks ago by ATpoint44k
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blast+ v2.10.1. error Error: mdb_env_open: Cannot allocate memory
... hi everyone! i am using blast+ to make db, but i one error command line: ncbi-blast-2.10.1+/bin/makeblastdb -in plasmid_refseq.fasta -dbtype nucl -parse_s error: Keep MBits: T Maximum file size: 1000000000B No volumes were created. Error: mdb_env_open: Cannot allocate m ...
software error alignment sequencing written 3 months ago by MEITUO10 • updated 3 months ago by GenoMax94k
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Comment: C: Segmentaton fault with ART for simulating paried-reads
... thnaks, i will try to look at it ...
written 3 months ago by MEITUO10
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Comment: C: Segmentaton fault with ART for simulating paried-reads
... i had used randomreads.sh, but it is quite slow if i want to get 10,000,000 reads, so i try to use other tools, whether these are more quickly ...
written 3 months ago by MEITUO10
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Segmentaton fault with ART for simulating paried-reads
... ART/art_bin_MountRainier/art_illumina -ss HS25 -sam -i precision_dataset.fna -l 150 -f 1 -o art-paired-dat ====================ART==================== ART_Illumina (2008-2016) Q Version 2.5.8 (June 7, 2016) Contact: Weichun Hu ...
next-gen sequencing written 3 months ago by MEITUO10 • updated 3 months ago by Mensur Dlakic8.1k
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Comment: C: kegg enrichment and pathway analysis
... hi! the input file of DAVID is a gene list, is the gene list in the emapper.annotations file? or how can i get the gene list? ...
written 3 months ago by MEITUO10

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