User: rob234king
rob234king • 600
- Reputation:
- 600
- Status:
- Trusted
- Location:
- UK/Harpenden/Rothamsted Research
- Website:
- http://elvis.misc.cran...
- Last seen:
- 6 months, 1 week ago
- Joined:
- 7 years, 9 months ago
- Email:
- r*********@gmail.com
Cranfield University MSc Applied Bioinformatics, Queen Mary University of London PhD Cancer Radiopharmaceuticals. Employed at Rothamsted Research.
Posts by rob234king
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... I am trying to submit a chromsome level genome using AGP file which passes validation externally but when use the ENA webin jar it complains. I think I am missing something obvious? The 6th column is just made up names by a script is that wrong?
ERROR: The component does not exist: "contig1_0". ...
written 7 months ago by
rob234king • 600
• updated
5 months ago by
Biostar ♦♦ 20
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C: submit Mitochondria to ENA
... Yes solved it, but still got the abutting gene error that they say they fixed in 2.2.4 but ill contact them to check they have fixed it. ...
written 8 months ago by
rob234king • 600
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Comment:
C: submit Mitochondria to ENA
... that might be it, I have not listed the chromosome list file in the manifest. I'll give that a go. ...
written 8 months ago by
rob234king • 600
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... ./ascp -D -v -i ../etc/asperaweb_id_dsa.openssh -L- -QT -l 300m UK99_R1.fastq.gz Webin-XXX@webin.ebi.ac.uk:.
solved it by just removing the -P part ...
written 8 months ago by
rob234king • 600
• updated
8 months ago by
GenoMax ♦ 94k
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... is it up and running as I'm having the same problem ...
written 8 months ago by
rob234king • 600
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1
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Comment:
C: submit Mitochondria to ENA
... I tried that but still no joy. I'll have to go through again line by line and check all details again, maybe i'm missing something obvious. Would be good if there was an actual human readable error to these things. ...
written 8 months ago by
rob234king • 600
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Comment:
C: submit Mitochondria to ENA
... Mt MIT chromosome Mitochondrion ...
written 8 months ago by
rob234king • 600
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... submitting data to ENA via aspera. I keep getting blocked error. even though checked with IT and firewall looks fine. XXX has my username. Any ideas what I am missing on the command
./ascp -D -v -i ../etc/asperaweb_id_dsa.openssh -QT -P 33001 -l 300m 1.fastq.gz Webin-XXXXX@webin.ebi.ac.uk:.
...
written 8 months ago by
rob234king • 600
0
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Comment:
C: submit Mitochondria to ENA
... I did a dos2unix on manifest file and embl file but no change. Also checked spaces. so irritating. ...
written 8 months ago by
rob234king • 600
0
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Comment:
C: submit Mitochondria to ENA
... I could just take out the correct translation table and let it go through as 1 but that would mean incorrect translations ...
written 8 months ago by
rob234king • 600
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