User: rob234king

gravatar for rob234king
rob234king600
Reputation:
600
Status:
Trusted
Location:
UK/Harpenden/Rothamsted Research
Website:
http://elvis.misc.cran...
Last seen:
1 month ago
Joined:
7 years, 4 months ago
Email:
r*********@gmail.com

Cranfield University MSc Applied Bioinformatics, Queen Mary University of London PhD Cancer Radiopharmaceuticals. Employed at Rothamsted Research.

Posts by rob234king

<prev • 183 results • page 1 of 19 • next >
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AGP file ENA submission error
... I am trying to submit a chromsome level genome using AGP file which passes validation externally but when use the ENA webin jar it complains. I think I am missing something obvious? The 6th column is just made up names by a script is that wrong? ERROR: The component does not exist: "contig1_0". ...
ena written 10 weeks ago by rob234king600 • updated 7 days ago by Biostar ♦♦ 20
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Comment: C: submit Mitochondria to ENA
... Yes solved it, but still got the abutting gene error that they say they fixed in 2.2.4 but ill contact them to check they have fixed it. ...
written 3 months ago by rob234king600
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Comment: C: submit Mitochondria to ENA
... that might be it, I have not listed the chromosome list file in the manifest. I'll give that a go. ...
written 3 months ago by rob234king600
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Answer: A: uploading using aspera to ENA raw data
... ./ascp -D -v -i ../etc/asperaweb_id_dsa.openssh -L- -QT -l 300m UK99_R1.fastq.gz Webin-XXX@webin.ebi.ac.uk:. solved it by just removing the -P part ...
written 3 months ago by rob234king600 • updated 3 months ago by genomax87k
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Comment: C: ERROR: "ascp: Failed to open TCP connection for SSH, exiting."
... is it up and running as I'm having the same problem ...
written 3 months ago by rob234king600
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Comment: C: submit Mitochondria to ENA
... I tried that but still no joy. I'll have to go through again line by line and check all details again, maybe i'm missing something obvious. Would be good if there was an actual human readable error to these things. ...
written 3 months ago by rob234king600
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Comment: C: submit Mitochondria to ENA
... Mt MIT chromosome Mitochondrion ...
written 3 months ago by rob234king600
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uploading using aspera to ENA raw data
... submitting data to ENA via aspera. I keep getting blocked error. even though checked with IT and firewall looks fine. XXX has my username. Any ideas what I am missing on the command ./ascp -D -v -i ../etc/asperaweb_id_dsa.openssh -QT -P 33001 -l 300m 1.fastq.gz Webin-XXXXX@webin.ebi.ac.uk:. ...
data ena written 3 months ago by rob234king600
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Comment: C: submit Mitochondria to ENA
... I did a dos2unix on manifest file and embl file but no change. Also checked spaces. so irritating. ...
written 3 months ago by rob234king600
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Comment: C: submit Mitochondria to ENA
... I could just take out the correct translation table and let it go through as 1 but that would mean incorrect translations ...
written 3 months ago by rob234king600

Latest awards to rob234king

Scholar 3 months ago, created an answer that has been accepted. For A: uploading using aspera to ENA raw data
Popular Question 3 months ago, created a question with more than 1,000 views. For R/perl/python script to bin scaffold coverage by cM for many samples
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Popular Question 20 months ago, created a question with more than 1,000 views. For start codon gff annotation corrections using fasta file
Teacher 20 months ago, created an answer with at least 3 up-votes. For A: Workflow Or Tutorial For Snp Calling?
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