User: diwasri

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Comment: C: Demultiplex Novaseq run with mixed samples containing either UDI alone or UMI-UD
... Thank you. So, to clarify, I will need to have separate sample sheets for the samples with UDI only and for those with the xGen UDI-UMI and then process them separately. For the UDI only samples, can I use the code originally mentioned with the exception of the --use-bases-mask changed to Y51,I8,I8 ...
written 4 weeks ago by diwasri0
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Demultiplex Novaseq run with mixed samples containing either UDI alone or UMI-UDI
... I have the raw basecall files from a Novaseq run that was run using the XP workflow. In each of the lanes I have 8 samples with UDI only and 8 samples with xGen UMI-UDI. I need to demultiplex the samples and generate fastq files. The command I am trying to use is /root/bin/bcl2fastq --input-di ...
umi rna-seq demultiplexing written 4 weeks ago by diwasri0 • updated 4 weeks ago by genomax91k

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