User: microfuge

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microfuge1.3k
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Posts by microfuge

<prev • 235 results • page 1 of 24 • next >
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When to ask for rerun from vendors
... Dear All, I was wondering about the thumb rules used to flag a concern to sequencing data vendors. Previously we had sample swaps where the library types were swapped and they did the rerun for free. But when to draw a line for per base quality or tile failure. Some thumb rule like if x percent of ...
ngs vendor written 7 days ago by microfuge1.3k
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Comment: C: Difference between variant calling and genotyping
... another absolute gem from Kevin ...
written 14 days ago by microfuge1.3k
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Comment: C: Difference between variant calling and genotyping
... my guess would be variant calling =~ probing for existence of alleles and genotyping ~= finding which alleles (allelic pair in diploids) exist in an individual. ...
written 14 days ago by microfuge1.3k
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Comment: C: samtools mpileup: bam file to consensus sequence generation error?
... There is an issue on github related to his https://github.com/samtools/bcftools/issues/391 ...
written 16 days ago by microfuge1.3k
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Answer: C: Snoscan#error#make: Nothing to be done for 'all'
... That would usually mean the target exists. Typically you can do a `make clean` and then a `make` to start compiling. ...
written 16 days ago by microfuge1.3k
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Comment: C: appropriate ticketing system for bioinformatians
... Thanks so much Emily for your detailed and thoughtful reply. ...
written 17 days ago by microfuge1.3k
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appropriate ticketing system for bioinformatians
... Hello, I would be grateful for advice on good ticketing systems for bioinfo support. Do standard IT ticket systems like Fork8 etc are appropriate to use in genomics or. Earlier I used gitlab issues but the clients also needed to have a gitlab account. I can think of a few requirements but please f ...
ticketing written 17 days ago by microfuge1.3k • updated 17 days ago by Carambakaracho1.6k
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Comment: C: How to retrieve protein sequence data for humano in UniProt?
... You can use the advance search option with selecting taxonomy from dropdown and then putting in 9606 (the taxonomy id of human) The autocomplete should suggest accordingly. ...
written 17 days ago by microfuge1.3k
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Comment: C: HOW TO remove all spaces except first space with semicolon
... This perl one liner could work `perl -lane '{printf ((shift @F)." ");print join(";",@F) }' your_input_file` or with sed skipping the first match `sed -s 's/\s\+/;/2g' your_input_file` ...
written 18 days ago by microfuge1.3k
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Answer: C: bcftools mpileup -r flag
... 2L should mean the chromosome/contig name. Needs to be substituted with the contig name where you want to call SNPS. I think that omitting the coordinates would do the analysis for the entire chromosome/contig. ...
written 20 days ago by microfuge1.3k

Latest awards to microfuge

Voter 21 days ago, voted more than 100 times.
Commentator 28 days ago, created a comment with at least 3 up-votes. For C: Fasta alignment to Tajima's D and population statistics
Commentator 6 weeks ago, created a comment with at least 3 up-votes. For C: Fasta alignment to Tajima's D and population statistics
Scholar 6 months ago, created an answer that has been accepted. For C: BedTools getfasta -split
Guru 6 months ago, received more than 100 upvotes.
Teacher 8 months ago, created an answer with at least 3 up-votes. For A: chain file for maize
Teacher 11 months ago, created an answer with at least 3 up-votes. For A: chain file for maize
Scholar 15 months ago, created an answer that has been accepted. For A: Many SRR files for one experiment. What are the steps to be taken before further
Teacher 15 months ago, created an answer with at least 3 up-votes. For A: chain file for maize
Scholar 15 months ago, created an answer that has been accepted. For A: Many SRR files for one experiment. What are the steps to be taken before further
Teacher 15 months ago, created an answer with at least 3 up-votes. For A: chain file for maize
Scholar 15 months ago, created an answer that has been accepted. For A: Many SRR files for one experiment. What are the steps to be taken before further
Scholar 16 months ago, created an answer that has been accepted. For A: Many SRR files for one experiment. What are the steps to be taken before further
Commentator 18 months ago, created a comment with at least 3 up-votes. For C: Fasta alignment to Tajima's D and population statistics
Popular Question 20 months ago, created a question with more than 1,000 views. For Tissue Specificity index (tau) should FPKM data be log transformed?
Scholar 2.1 years ago, created an answer that has been accepted. For A: Many SRR files for one experiment. What are the steps to be taken before further
Teacher 2.1 years ago, created an answer with at least 3 up-votes. For A: chain file for maize
Scholar 2.2 years ago, created an answer that has been accepted. For A: Many SRR files for one experiment. What are the steps to be taken before further
Scholar 2.2 years ago, created an answer that has been accepted. For A: Many SRR files for one experiment. What are the steps to be taken before further
Teacher 2.2 years ago, created an answer with at least 3 up-votes. For A: chain file for maize
Commentator 2.9 years ago, created a comment with at least 3 up-votes. For C: Fasta alignment to Tajima's D and population statistics
Supporter 2.9 years ago, voted at least 25 times.
Teacher 2.9 years ago, created an answer with at least 3 up-votes. For A: chain file for maize
Centurion 3.0 years ago, created 100 posts.
Commentator 3.0 years ago, created a comment with at least 3 up-votes. For C: Fasta alignment to Tajima's D and population statistics

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