User: stolarek.ir

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stolarek.ir640
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Poland
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Posts by stolarek.ir

<prev • 119 results • page 1 of 12 • next >
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PacBio base modifications merging p6c2 and p4c2
... When searching for modified bases in PacBio sequencing, can I merge datasets coming from 2 different polymerases: P6C2 and P4C2 to increase the coverage? Or will the pipeline be misled by such an attempt? ...
base modifications pacbio written 8 months ago by stolarek.ir640 • updated 8 months ago by richard.james.hall20
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Comment: C: Merging files in plink
... you can filter by the genotyping rate with the --geno flag ...
written 21 months ago by stolarek.ir640
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Answer: A: ABBA-BABA D statistics to pattern introgressed loci: applicable to RNA-seq data?
... vast majority of such variants likely originated in the genome, so I would say, no problem, but there is nothing inherent to transcriptome here ...
written 21 months ago by stolarek.ir640
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Answer: A: STAR analysis and alignment
... there is option --sparse which reduces the RAM needs to 16 GB for human genome ...
written 22 months ago by stolarek.ir640
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Comment: C: Illumina paired end read header difference- SPAdes bwa run ERROR
... download bbmap and use repair.sh from it like so: ./repair.sh in1=pair1.fastq in2=pair2.fastq out1=out_pair1.fastq out2=out_pair2.fastq outsingle=out_single.fastq ...
written 2.2 years ago by stolarek.ir640
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Comment: C: RNASeq lane effect
... there might be something off if you multiplex hardly, and the indexes used within the lane do not co-op with each other well ...
written 2.2 years ago by stolarek.ir640
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Comment: C: long reads Pacbio SNP calling
... either look at their webpage if they have anything dedicated for that, or use GATK ...
written 2.3 years ago by stolarek.ir640
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Answer: A: long reads Pacbio SNP calling
... no worries, with high coverage you can do a decent SNP calling using PacBio ...
written 2.3 years ago by stolarek.ir640
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codeml, output tree topology different from the input tree
... Dear all, I am struggling with understanding the output of the codeml. I used typical M0 model with settings like so: runmode = 0 seqtype = 1 CodonFreq = 2 clock = 0 model = 0 NSsites = 0 The provided tree (runmode = 0) is however severely altered in the o ...
paml codeml written 2.3 years ago by stolarek.ir640
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Comment: C: Paired vs unpaired (DNA Sequencing)
... cmon, at least use google and read about anything on the first page ...
written 2.3 years ago by stolarek.ir640

Latest awards to stolarek.ir

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Scholar 2.0 years ago, created an answer that has been accepted. For A: PLINK RECODING ISSUE
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