User: leipinji

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leipinji10
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10
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New User
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Last seen:
1 year ago
Joined:
6 years, 6 months ago
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l*******@gmail.com

I am a student in WuHan University China

Posts by leipinji

<prev • 9 results • page 1 of 1 • next >
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The way to compare ChIPSeq peaks enrichment level in different samples
... I have ChIPSeq data from four samples , and I want to compare peaks enrichment level among those samples.  ...
chip-seq written 4.4 years ago by leipinji10 • updated 2.9 years ago by Biostar ♦♦ 20
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Comment: C: Counting number of genes using htseq-count tool.
... yes, samtoool sort by name will sort paired end reads forward and reverse together.  ...
written 4.4 years ago by leipinji10
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Comment: C: Counting number of genes using htseq-count tool.
... If you used paired end data , bam file should be sorted by name. samtools sort -n in.bam out.srt ...
written 4.4 years ago by leipinji10 • updated 3 months ago by RamRS25k
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Comment: C: Anyone knowns piRNA sequencing analysis strategy?
... Sorry, because of internet problem, I submitted this question twice.  ...
written 4.4 years ago by leipinji10
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Anyone knowns piRNA sequencing analysis strategy?
... We have done piRNA-Seq, and for data analysis we meet some trouble. The wet lab pipeline: 1.We focus on three proteins and we want to known if those proteins can sepcificly interact with piRNAs. 2.tissue came from mouse testis were used for RNA immunoprecipitation. 3.after RIP, piRAN was selected by ...
miran-seq ripseq rna-seq piran written 4.4 years ago by leipinji10
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(Closed) Anyone knowns piRNA sequencing analysis strategy?
... We have done piRNA-Seq, and for data analysis we meet some trouble. The wet lab pipeline: 1.We focus on three proteins and we want to known if those proteins can sepcificly interact with piRNAs. 2.tissue came from mouse testis were used for RNA immunoprecipitation. 3.after RIP, piRAN was selected by ...
miran-seq ripseq rna-seq piran written 4.4 years ago by leipinji10
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Comment: C: RNASeq normalization using the expression values of rRNA
... But many people use FPKM for RNASeq gene expression level quantification. Could you please explain why shouldn't we use FPKM value for normalization? ...
written 4.4 years ago by leipinji10
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Answer: A: RNASeq normalization using the expression values of rRNA
... I think you can not use rRNA for normalization. Firstly, for RNASeq library construction we first purify mRNA from total RNA and then sequencing those mRNA fragments. And the purification step will remove most rRNA. Secondly, if you used total RNA for library construction, the rate of mRNA is only 2 ...
written 4.4 years ago by leipinji10
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Why Are There Many Mirna Or Noncoding Rna In My Mrna-Seq Data?
... I'm a freshman in bioinformatics, and when I do the RNA-seq data analysis I found many miRNA or noncoding RNA in my results, why? ...
rna-seq mirna written 6.5 years ago by leipinji10 • updated 6.5 years ago by Michael Dondrup47k

Latest awards to leipinji

Popular Question 19 months ago, created a question with more than 1,000 views. For The way to compare ChIPSeq peaks enrichment level in different samples
Popular Question 4.3 years ago, created a question with more than 1,000 views. For The way to compare ChIPSeq peaks enrichment level in different samples
Popular Question 4.3 years ago, created a question with more than 1,000 views. For Why Are There Many Mirna Or Noncoding Rna In My Mrna-Seq Data?
Popular Question 4.3 years ago, created a question with more than 1,000 views. For Anyone knowns piRNA sequencing analysis strategy?

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