User: Matina

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Matina170
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United Kingdom/University of Edinburgh
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3 months ago
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Posts by Matina

<prev • 39 results • page 1 of 4 • next >
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Comment: C: TCGA RNA-Seq: are RSEM or Z-scores based expression comparable to each other?
... Hi, by "comparing expression levels between sample groups" you mean differential expression analysis? ...
written 8 months ago by Matina170
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Comment: C: TCGAbiolinks TCGA-BRCA RNA-seq clinical data
... Hi Atakan, This is correct - I got an answer from one of the developers of TCGAbiolinks at the [Github account][1] saying that everything that starts with `subtype_` is actually metadata from papers that analyzed the samples suggested to use `days_to_death`. In any case what is strange is that the ...
written 18 months ago by Matina170
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Comment: C: TCGAbiolinks TCGA-BRCA RNA-seq clinical data
... Thank you very much Igor! I will have a look at this! ...
written 18 months ago by Matina170
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Comment: C: TCGAbiolinks TCGA-BRCA RNA-seq clinical data
... Hi Efstathios, I have a set of genes that I am interested in and I want to see if they are associated with clinical outcomes and molecular subtype patterns. You are right, I got an answer in the GitHub account. Thanks a lot for your answer! Matina ...
written 18 months ago by Matina170
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TCGAbiolinks TCGA-BRCA RNA-seq clinical data
... Hi all, I have downloaded the TCGA-BRCA RNA-seq data and the associated clinical information using the code below. CancerProject <- "TCGA-BRCA" query <- GDCquery(project = CancerProject, data.category = "Transcriptome Profiling", dat ...
tcgabiolinks tcga brca rna-seq written 18 months ago by Matina170 • updated 18 months ago by igor8.9k
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Comment: C: Similar samples in RNAseq
... Have you looked at Pearson correlation between the samples of interest? ...
written 19 months ago by Matina170
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Answer: A: PAM50 subtype classification by genefu
... Hi vinayjrao, I think the problem is with the `data` matrix; `molecular.subtyping` fuction expects a matrix of samples(rows) x genes(cols). As I can see above your data matrix is genes x samples, right? Try transposing the matrix. From the genefu vignette for do.mapping: `TRUE if the mapping thro ...
written 19 months ago by Matina170
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Comment: C: PAM50 subtype classification by genefu
... Great! Happy to help! I'll post it as an answer then. ...
written 19 months ago by Matina170
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Comment: C: PAM50 subtype classification by genefu
... Hi vinayjrao, I think the problem is with the `data` matrix; `molecular.subtyping` fuction expects a matrix of samples(rows) x genes(cols). As I can see above your data matrix is genes x samples, right? Try transposing the matrix. Let me know if it solved your problem! From the genefu vignette fo ...
written 19 months ago by Matina170
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Comment: C: Differential Expression from Quantile-Norm Data
... Ok, so have you tried using lets say EdgeR or DESeq2 with the raw read counts? If you would prefer to use the normalised data you could use a limma-voom solution. As for GSEA, after you determine differentially expressed genes between the groups of interest you can rank the genes based on some crite ...
written 19 months ago by Matina170

Latest awards to Matina

Popular Question 8 months ago, created a question with more than 1,000 views. For Ballgown plotTranscripts function
Popular Question 8 months ago, created a question with more than 1,000 views. For Ballgown plotTranscripts function
Popular Question 15 months ago, created a question with more than 1,000 views. For Ballgown plotTranscripts function
Supporter 18 months ago, voted at least 25 times.
Scholar 19 months ago, created an answer that has been accepted. For A: PAM50 subtype classification by genefu
Popular Question 2.2 years ago, created a question with more than 1,000 views. For Ballgown plotTranscripts function
Popular Question 2.2 years ago, created a question with more than 1,000 views. For Uninstall samtools 1.1 version
Popular Question 3.3 years ago, created a question with more than 1,000 views. For Uninstall samtools 1.1 version
Popular Question 3.7 years ago, created a question with more than 1,000 views. For EdgeR analysis with 3 conditions
Student 3.8 years ago, asked a question with at least 3 up-votes. For EdgeR-GLMs correcting for batch effects
Popular Question 5.0 years ago, created a question with more than 1,000 views. For EdgeR-GLMs correcting for batch effects

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