User: prp291

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prp29150
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Posts by prp291

<prev • 32 results • page 1 of 4 • next >
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Comment: C: Combining the RNAseq datasets
... actually I want to identify the tissue specific genes in my plants. 100bp and 50bp datasets cover different kind of tissue. So my goal is to mixed of both datasets and then identify the tissue specific genes. Thanks. ...
written 22 months ago by prp29150
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Comment: C: Combining the RNAseq datasets
... Thanks for comments. I am using a plant genome. Both 50bp and 100bp showed more than 70 % alignment rate. ...
written 22 months ago by prp29150
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Comment: C: Combining the RNAseq datasets
... I think this answer was not intended for this question. ...
written 22 months ago by prp29150
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Comment: C: Combining the RNAseq datasets
... Thanks for the links. I think situation is little bit different in my case. In my case both datasets are using the technique (RNAseq) but read length is different. I was wondering whether difference in read length will me any impact on final result? ...
written 2.0 years ago by prp29150
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Combining the RNAseq datasets
... I have a RNAseq experiment data (50bp) into two tissue. I want to combine it with previously reported RNAseq data (100bp) to analyze the gene expression in different tissue. Can I combine both data together or I should go through some kind of normalization? Any insight will be helpful. Thanks ...
next-gen rna-seq written 2.0 years ago by prp29150 • updated 23 months ago by theobroma221.1k
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Plant pathogen interaction RNAseq analysis
... Hi, Yesterday I [post a question][1] about different mapping percentage for control and treated (virus infected) plant. Later I found that about 25% of my reads from treated libraries are mapping with the virus genome I used for infection that leads the discrepancy in mapping rate. My question is ho ...
rna-seq written 2.9 years ago by prp29150
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Comment: C: Different mapping rate problem
... I didn't use the rib-depletion methods for library preparation therefore I assume that I have rRNA sequence there. ...
written 2.9 years ago by prp29150
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Comment: C: Different mapping rate problem
... Thanks. How to get rid of them? ...
written 2.9 years ago by prp29150
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Comment: C: Different mapping rate problem
... tHANKS FOR YOUR HELP. all libraries have Q30 more than 97%. They also passed all parameters of fastqc except the kmer. I will try to BLAST the unmapped reads. ...
written 2.9 years ago by prp29150
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Comment: C: Different mapping rate problem
... Thanks. Can you point out some probable cause of this discrepancy? ...
written 2.9 years ago by prp29150

Latest awards to prp291

Popular Question 22 months ago, created a question with more than 1,000 views. For Bowtie2 low mapping percentage
Popular Question 22 months ago, created a question with more than 1,000 views. For Get specific elements from clustered data in R
Popular Question 23 months ago, created a question with more than 1,000 views. For Bowtie2 low mapping percentage
Great Question 2.6 years ago, created a question with more than 5,000 views. For Extract Fasta Sequences Sub Sets
Popular Question 2.6 years ago, created a question with more than 1,000 views. For Bowtie2 low mapping percentage
Popular Question 2.6 years ago, created a question with more than 1,000 views. For Eukaryotic genome annotation steps
Popular Question 3.3 years ago, created a question with more than 1,000 views. For Extract Fasta Sequences Sub Sets
Popular Question 5.1 years ago, created a question with more than 1,000 views. For Extract Fasta Sequences Sub Sets

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