Question: What are the possible sources of sequence contamination in different sequencing approaches?
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gravatar for bulovic.ana
4.9 years ago by
bulovic.ana70
Croatia
bulovic.ana70 wrote:

I'm interested what biological contaminants can effect the net result of sequencing.

I've read some things about it (like this here: http://www.ncbi.nlm.nih.gov/tools/vecscreen/contam/), but have no experience as to when the different sources of contamination are expected. For instance, it states that "DNA/cDNAs from the biological source organism/organelle are usually inserted into a cloning vector".

What does "usually" signify? What is the alternative?

How do the biological contamination sources differ in the different sequencing approaches (genome / transcriptome / exome)?

Is there some online resource where I could get an idea of all the steps involved? I've watched a couple of videos and they are ~5 minute explanations, but vague on the details. I would like a rather comprehensive and detailed guide.

sequencing contamination • 1.2k views
ADD COMMENTlink modified 4.9 years ago • written 4.9 years ago by bulovic.ana70
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