Sequence identity in syngenic region (mouse, human), pairwise alignment
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Entering edit mode
9.7 years ago

Hi,

I have a question which hopefully is easy to answer for you (but hard for me ;-) )

I'm interested in a specific genomic region in mouse (about 6Mb in total) that has large stretches of non-coding regions. As I identified a function to a certain part of one of the non-coding regions I'd like to score it's conservation in humans (the whole region is quite conserved as I visualized it in VISTA). Thus I thought about % sequence identity upon pairwise alignment and would like to put it in relation to the sequence identity in the other parts surrounding my specific region (and overall human/mouse identity). But now I'm stuck as I'm not a bioinformatician at all and relatively new to these "genomic" analyses. Is there an "easy" way to do that (do I get this info e.g. with VISTA already)?

Thanks a lot in advance :-)

Nina

sequence alignment genome • 1.7k views
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