Doubts regarding phylogeny analysis with protein sequence
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9.6 years ago
lia.elias ▴ 40

1. While making a phylogenetic tree with a protein sequence is it ok if I include sequences inferred from homology?

In the uniport, there are protein sequences of 4 categories: those that have evidence at protein level, transcript level, derived from homology and just predicted sequences. My question is if I include the sequences inferred from homology to the analysis, will it affect the reliability of the tree and ancestor sequence?

2. After Clustal analysis and MEGA analysis how can I obtain the probable ancestor protein sequence?

3. While going for the determination of ancestor sequence and/or consensus sequence can we include the outgroup too?

sequence • 1.5k views
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