Entering edit mode
11.0 years ago
seta
★
1.9k
Hi everybody,
The E-value cutoff in the miRBase is 10 by default. In my opinion,it seems a bit high for miRNA (short sequence) since the probability of having good alignment by chance is increased as the sequence length is decreased. If you agree with me, could you please let me know on the basis of E-value, which hits resulted from blast output should be considered for conducting further analysis?