What Gene Information Do I Need To Screen The Gene Ontology With David?

Question

[Amod Kuamr]

I have list of genes, and want to analyse these genes for immune related gene ontology terms with DAVID. How should I identify my selection of genes and what further information do I need to add, to prepare input for DAVID?

[Sean Davis]

You would normally just paste your gene list into the gene list box on the website. Perhaps you can clarify what your question is?

[Larry_Parnell]

A list of gene symbols - to be precise - will do the trick. Gene names will not give interpretable results. Official Symbol: PRKACA. Name: protein kinase, cAMP-dependent, catalytic, alpha.

Answer 1

[Sean Davis]

DAVID does not deal with miRNAs as far as I know. I do not know of a tool that analyzes miRNAs with respect to GO since I do not think that miRNAs are annotated in GO.

[Amod Kuamr]

sir, i mean that i have genes list, that list i got by miRNA target prediction...

Answer 2

[Manish]

To SEAN DAVIS, "Identification of differentially expressed miRNAs in chicken lung and trachea with avian influenza virus infection by a deep sequencing approach" BMC Genomics 2010, 11:373doi:10.1186/1471-2164-11-373"

this article contain enriched immune related GO terms of list of genes. How can it be achieved?

[Sean Davis]

Normally, when clarifying your question, you would do that as a comment. It still is not clear what you have. Do YOU have a list of genes that is of interest to you, for example, from a microarray experiment where you looked for differentially-expressed genes?

[Sean Davis]

Go to the DAVID website, click on "Gene Functional Classification" and then paste your gene list in the text box. Then, follow the steps outlined on the site.

[Amod Kuamr]

sir i have target genes of miRNA. i want to do GO analysis by DAVID and want enriched immune related GO terms. so i can do it.

[Amod Kuamr]

sir i have target genes of miRNA. i want to do GO analysis by DAVID and want enriched immune related GO terms. how can i do it. please make me understand by an example.