Question: What Gene Information Do I Need To Screen The Gene Ontology With David?
0
gravatar for Amod Kuamr
8.0 years ago by
Amod Kuamr0
Amod Kuamr0 wrote:

I have list of genes, and want to analyse these genes for immune related gene ontology terms with DAVID. How should I identify my selection of genes and what further information do I need to add, to prepare input for DAVID?

gene • 2.1k views
ADD COMMENTlink modified 7.8 years ago by Sean Davis25k • written 8.0 years ago by Amod Kuamr0
1

You would normally just paste your gene list into the gene list box on the website. Perhaps you can clarify what your question is?

ADD REPLYlink written 8.0 years ago by Sean Davis25k

A list of gene symbols - to be precise - will do the trick. Gene names will not give interpretable results. Official Symbol: PRKACA. Name: protein kinase, cAMP-dependent, catalytic, alpha.

ADD REPLYlink written 7.9 years ago by Larry_Parnell16k
1
gravatar for Sean Davis
8.0 years ago by
Sean Davis25k
National Institutes of Health, Bethesda, MD
Sean Davis25k wrote:

DAVID does not deal with miRNAs as far as I know. I do not know of a tool that analyzes miRNAs with respect to GO since I do not think that miRNAs are annotated in GO.

ADD COMMENTlink written 8.0 years ago by Sean Davis25k

sir, i mean that i have genes list, that list i got by miRNA target prediction...

ADD REPLYlink written 8.0 years ago by Amod Kuamr0
0
gravatar for Manish
8.0 years ago by
Manish0
Manish0 wrote:

To SEAN DAVIS, "Identification of differentially expressed miRNAs in chicken lung and trachea with avian influenza virus infection by a deep sequencing approach" BMC Genomics 2010, 11:373doi:10.1186/1471-2164-11-373"

this article contain enriched immune related GO terms of list of genes. How can it be achieved?

ADD COMMENTlink written 8.0 years ago by Manish0
2

Normally, when clarifying your question, you would do that as a comment. It still is not clear what you have. Do YOU have a list of genes that is of interest to you, for example, from a microarray experiment where you looked for differentially-expressed genes?

ADD REPLYlink written 8.0 years ago by Sean Davis25k
1

Go to the DAVID website, click on "Gene Functional Classification" and then paste your gene list in the text box. Then, follow the steps outlined on the site.

ADD REPLYlink written 8.0 years ago by Sean Davis25k

sir i have target genes of miRNA. i want to do GO analysis by DAVID and want enriched immune related GO terms. so i can do it.

ADD REPLYlink written 8.0 years ago by Amod Kuamr0

sir i have target genes of miRNA. i want to do GO analysis by DAVID and want enriched immune related GO terms. how can i do it. please make me understand by an example.

ADD REPLYlink written 8.0 years ago by Amod Kuamr0
Please log in to add an answer.

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 1932 users visited in the last hour