Hi I am trying to use a small RNA data from CD34 bone marrow cells to compare with another private data that I have.
I just downloaded it from SRA (sra id: SRR772115) and converted it using fastq-dump. But the results don't look typical of a fastq file. Since each read in fastq file is represented in 4 lines while here its not the case. Here is how the converted fastq file looks like.
@SRR772115.1 FCC0B8BACXX:8:1101:1416:2034 length=49 TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGATCTCGTATG +SRR772115.1 FCC0B8BACXX:8:1101:1416:2034 length=49 bbbeeeeeggggghhiiiiihiiiiiiiiggfhicfghihhiiihhhii @SRR772115.2 FCC0B8BACXX:8:1101:1317:2047 length=49 TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGTGGATCTCGTATG +SRR772115.2 FCC0B8BACXX:8:1101:1317:2047 length=49 bbbeeeeegggggghiiiiiiiiiiiiiiihiiicgghhghhiiiihih @SRR772115.3 FCC0B8BACXX:8:1101:1437:2047 length=49 TATGGTCGCAAGGCTGAAACTTAAAGAAATTGATGGAATTCTCGGGTGC
Can anybody tell me if I am missing something here. And how to get the fastq in proper format.