Question: Two Fastq Files And Alignment With Bwa.
1
gravatar for KCC
7.3 years ago by
KCC3.9k
Cambridge, MA
KCC3.9k wrote:

I have two fastq files. I want to use bwa to produce two sam files as this is more efficent for me than merging the fastq files and running bwa on the bigger file. If I run bwa separately on the fastq files and merge the sam files later, will the result be different? (I don't know if this is an important detail, but I know a priori that the fastq files have reads from separate chromosomes.)

fastq alignment sam bwa • 3.3k views
ADD COMMENTlink modified 12 months ago by Biostar ♦♦ 20 • written 7.3 years ago by KCC3.9k
9
gravatar for Damian Kao
7.3 years ago by
Damian Kao15k
USA
Damian Kao15k wrote:

Individual reads are mapped independently of each other. So mapping the reads in two chunks should produce the same mappings as a single file. However, I wouldn't expect mapping runs to always be identical as there is a random element to mapping repetitive hits.

ADD COMMENTlink written 7.3 years ago by Damian Kao15k
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