How much of an issue is the fact that reads can be duplicates in RNA seq experiments?
Both single fragment and PE sequenced, is read duplication affecting a large proportion of the data? What are the duplicate rates like for an average human sample experiment? Is it in the order of 1% duplicates? Or 10% duplicates?
I have seen it mentioned in another question here in Biostars, I would like to get a feeling of how important this is in the field: