Question: Probe-Detection of Illumina HT-12 v4 Bead Arrays
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gravatar for Tobias.Wohland
3.7 years ago by
Germany
Tobias.Wohland60 wrote:

Hi,

I'm using the R-package lumi for the analyses of Illuminas HT-12 v4 expression chips. I currently have a problem with the probe detection. I want to use the mean of the negative control beads for probe detection. More precisely I consider a probe as detected which has a higher expression then 10% over the mean of the negative control beads. My problem is, which expression values should I use as template? I read that one should use the already background corrected expression values but I'm not 100% sure about it. What is your suggestion. Should I use the background corrected or the raw data? I mean the negative beads represent the background noise and if the raw expression values are higher then this (or my threshold: 10% over the mean of the negative control beads) then the transcript should be considered to be expressed. Please correct me if I'm wrong. Maybe I have a error in reasoning.

I used Model-Based Background Correction for my data.

Best,

Tobi

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