Differential Gene Expression Analysis with NimbleGen microarrays
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8.4 years ago
pixie@bioinfo ★ 1.5k

I am analyzing microarrays from the platform NimbleGen Arabidopsis thaliana 4x72K Array [080306_AT6_expr]

(http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GPL16226)

Is there any well-defined pipeline for its analysis to identify deferentially expressed genes using R package or any other tool ?

Kindly help.

microarray • 2.0k views
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8.4 years ago
morovatunc ▴ 550

You my check oligo R library for analysing Nimblegen data. It has quite well defined manual.

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Thank You, the paper for the dataset I am using also uses oligo as well as pdInfoBuilder (Falcon et al., 2012) packages using the AgilentAT6 build. I was wondering why they have used the pdInfoBuilder package.

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This is one of the most confusing things about that package. It creates such a "class" to annotate genes. For my case, I didn't have to chance to use annotation file that is downloaded from the database. Therefore, I had to create it manually. Hope your build is available online.

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