Question: Bwa What Is In .Sai File
1
gravatar for Sara
7.1 years ago by
Sara20
Sara20 wrote:

Hello,

I would like to know what is in .sai file after alignment with BWA. what should be remove before convert it to bam file

please advise thanks Sarm

bwa • 9.9k views
ADD COMMENTlink written 7.1 years ago by Sara20
6
gravatar for Damian Kao
7.1 years ago by
Damian Kao15k
USA
Damian Kao15k wrote:

Why do you want to remove anything from the .sai file? Why not convert it to .sam and then do your filtering?

.sai files are not a standardized file format and how bwa interprets/generates the files changes between versions of bwa.

I would convert it to .sam and not use the .sai files.

ADD COMMENTlink written 7.1 years ago by Damian Kao15k
4
gravatar for Fidel
7.1 years ago by
Fidel1.9k
Germany
Fidel1.9k wrote:

The .sai file is an intermediate file containing the suffix array indexes. Such file is afterwards translated into a SAM file.

The BWA paper may help you to understand how the mapping is done:

http://bioinformatics.oxfordjournals.org/content/25/14/1754.full

ADD COMMENTlink written 7.1 years ago by Fidel1.9k
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