All of the contigs from SOAPdenovo-trans are very short. I want to know the reason?
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8.9 years ago

Hello,

I used SOAPdenovo-trans to assemble one transcriptome. But the contigs are very short, like this:

>1 length 32 cvg_34.0_tip_0
CCACAGCGAGATCTGCCATTGGGAATCGCAAC
>3 length 32 cvg_22.0_tip_0
TCAAGGTTAGCCCTAATACTGTTGAGGGTTCC
>5 length 32 cvg_47.0_tip_0
CGACGTGCTCAAGGACGAGTACCCGGTGCCGG
>7 length 32 cvg_45.0_tip_1
ACATCTCGTTCTGGAGTTTTCGAGGGTGAGAA
>9 length 32 cvg_18.0_tip_1
GCTTCTGCTTGCAAACGTCCGGATGATACTGC
>11 length 32 cvg_63.0_tip_1
TTTGCAACAGCGTGGTCCTTTGCAAATTTGCA
>13 length 32 cvg_56.0_tip_0
GCTAAGAAGAGCTTAGCTTCTGATGAAGTGGC
>15 length 32 cvg_55.0_tip_1
CACCTTTTACGCTTTGATTTGAGGGTTGACGT
>17 length 32 cvg_46.0_tip_0
CCTGAAATGAAGGTGTTTCGAGAAATTTCCGT
>19 length 32 cvg_53.0_tip_0
TCTTCATTCTCAATCAAGGTTGGAGCAGTTAT

It's so strange. They are shorter than reads. I don't know why. Please tell me if you know the reason. Thank you very much.

Hui

RNA-Seq Assembly • 1.6k views
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