I was wondering if there is an experimental method to count the number of bound transcription factors on a promoter?
So, for example, you have a promoter/enhancer region with 5 transcription factor binding sites (TFBS), and you know that not all TFBS need to occupied to initiate transcription. How would one determine the mean number of occupied binding sites? And how for a promoter that has 2 TFBS, or for one that has 10 TFBS?
Thanks for your help,