Question: (Closed) Duplicate reads from Single read RNASeq
0
gravatar for ash
4.6 years ago by
ash0
United States
ash0 wrote:

Hi,

I am a bench scientist and I have a basic question. Can you remove duplicate reads from single read RNA-Seq data? If yes/no, could you please give me the reasoning?

Thank you!!

rna-seq single read duplicate • 1.1k views
ADD COMMENTlink written 4.6 years ago by ash0
2

Hello ash!

There are many many threads already discussed about duplicates and RNA-Seq.

Should We Remove Duplicated Reads In Rna-Seq ?
Duplicated Reads In Rna-Seq Experiment

http://seqanswers.com/forums/showthread.php?t=6854

Given that your data is single-end, its not recommended to remove duplicates. Please read the discussions in above posts.

For this reason we have closed your question. This allows us to keep the site focused on the topics that the community can help with.

If you disagree please tell us why in a reply below, we'll be happy to talk about it.

Cheers!

ADD REPLYlink modified 4.6 years ago • written 4.6 years ago by geek_y11k

You can, but that is an entirely different question to should you. There was actually a very good post about this 3 days ago which you can view here

Ironically, don't have the heart to mark a question about duplicates as a duplicate of a question about duplicates, but if you feel that the other post answers your question, you should consider moderating yourself :)

ADD REPLYlink modified 4.6 years ago • written 4.6 years ago by John12k
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