Question: Generating simulated gene expression data sample based on DESeq2 result
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gravatar for bharata1803
3.4 years ago by
bharata1803420
Japan
bharata1803420 wrote:

Hello,

I have a result of DESeq2 analysis of RNA-seq sample. In that result, it is mentioned about the baseMean and I think that baseMean is the mean of negative binomial distribution which is the distribution used by DESeq2. My question is, how to generate a sample following the distribution like used in DEseq2 with given mean from DESeq2? I don't know about the variation of the distribution though because it seems there are no variation variable there. Thank you.

rna-seq R • 1.1k views
ADD COMMENTlink modified 3.4 years ago by Biostar ♦♦ 20 • written 3.4 years ago by bharata1803420

So your goal is: given the results of a DESeq2 analysis, generate simulated sequence data which could have generated the given DESeq2 result?

ADD REPLYlink written 3.4 years ago by mbio.kyle330
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