Understanding MISO output

Question

[onspotproductions]

I recently ran MISO on some RNA-seq data as we are looking at isoform centric changes in levels. What I want to know is the best way to filter the data since there are multiple values in the Diff and bayes factor columns. Would it be best to create a percentage by simply adding the positive values and finding the largest ones (>50) or would it be better to look at the bayes values and find the average probability and limit the values equal to or greater than that? Any help is appreciated.

[Govardhan Anande]

Hi,

Which version and what command you using to run MISO on paired end data.

I am trying to run MISO but getting error "Waiting on n threads...", command I used "miso --run /indexed_SE_events/ Test.bam --output-dir Test/ --read-len 49 --paired-end=147 27 -p 10"

Thanks, Goa