I am doing a course project which I was asked to analyse RNAseq data. For this analysis I picked two data sets from GEO data base. The difference of these datasets is they have different read length. Dataset A has 50 sequence length and Dataset B has 202 sequence length. I obtained these values from FastQC software.
So I would like to know;
- My aim is to evaluate differentially expressed genes. Would it be logical to compare genes in these datasets?
- Should I use other softwares to evaluate sequence length? Also, forgive my ignorance about this question but is sequence length mean read length?
Thank you for your time, Best,