Question: Problem In Translating Nucleotide To Protein
0
gravatar for Shiva
7.1 years ago by
Shiva0
Shiva0 wrote:

Dear Friends,

I have following problems in the sequence analysis.

we have sequenced exon part of a specific gene that has lot of variations (insertions and deletions) due to which i am not able to translate into protien level. i have used expasy translation tool for the translation.It is giving different frames with stop codons.In all the frames i am finding stop codons an average 3-5.

please give me a solution.

thanking you.

translation protein • 1.5k views
ADD COMMENTlink written 7.1 years ago by Shiva0
1
gravatar for Peter Kovac
7.1 years ago by
Peter Kovac60
Bratislava, Slovakia
Peter Kovac60 wrote:

Can you be more specific about the gene (which organism etc.)? If there is some reference sequence for the organism, I'd try to make an alignment and look for possible frameshifts (if there is no reference sequence you can try ortholog alignment with similar species).

ADD COMMENTlink written 7.1 years ago by Peter Kovac60
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