microRNA seq vs RNA seq

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8.1 years ago

jolin0701-dy ▴ 100

I have 1 RNA seq and 1 microRNA seq.

As to analysis (QC and mapping), can we use the same softwares or different?

Should I download microRNA genomes for mapping?

Thanks~~

RNA-Seq • 2.2k views

ADD COMMENT • link 8.1 years ago by jolin0701-dy ▴ 100

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What do you think? How are they different (in terms of characteristics)?

ADD REPLY • link 8.1 years ago by GenoMax 142k

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The length is different..

ADD REPLY • link 8.1 years ago by jolin0701-dy ▴ 100

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How would you account for that in your QC (and subsequent mapping)? Will you need to trim and considering the different length would your strategy be different for the two sets? Would you map to the same database (or use something specialized in case of microRNA)?

ADD REPLY • link 8.1 years ago by GenoMax 142k

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I see, we should use different software…… thanks for your questions….. I'm a new beginner of rna seq and learning by myself now.. :P

ADD REPLY • link 8.1 years ago by jolin0701-dy ▴ 100

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