I noticed a high percentage of 3'UTR mutations human tumor RNAseq. Here is a paper (Fig.3) which shows < 10% of UTR mutations in their RNAseq data.
Is it unusual for such high percentage of UTR mutations? What could be some explanations? I greatly appreciate your feedback.
Here are my methods.
Tumor samples RNA extraction by TRIzol (Invitrogen) and the RNeasy kit (Qiagen), Illumina HiSeq 2000, 75bp pair-end
GATK RNAseq Best Practice - STAR-2pass with Gencode hg19 transcripts, SplitN'Trim, Indel Realignment, base recalibration.
Mutect2 with default params, keep "PASS" mutations, annotated with SNPEFF
Mutation Distributions from 32 tumor samples