Question: CRISPR screen 0 count values

1

morovatunc •

**400**wrote:Hi,

I would like to calculate simple log fold change between two conditions. However, I have 0 values which makes the calculation problematic. I have 4 sgRNA for a gene, counts refer to their values.

The problem is, I am ok with dealing the rows which have all zeros. But the problem is with the rows which have both. Therefore, I have concerns about what should be my method.

Any help would be appreciated,

Best,

Tunc.

Could you post an example of what the data looks like with the problem lines?

Would adding a small value to all the raw data (e.g. x+0.001) help with the log transformations?

1.2kDear Jotan thank you for your answer.

I solved the problem by adding +1 to all of the cells. Then take the ratio of two conditions. After that I took the log2.

My problem was like;

400