I have recently completed a de novo assembly for an organism whose genome is currently unknown. We are working to classify all the sequences, but would like to decode the ORFs so we can tie our data to data from MS. I have previosuly attempted to use transdecoder w/ homology search and emboss tools with not very accurate results. We have run the data against BLAST and obtained a high level of accurate results so we know our assembly is well put together. Any suggestions would be appreciated.
Question: Methods to identify ORF from de novo assembly
3.2 years ago by
onspotproductions • 130
onspotproductions • 130 wrote:
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