Question: Is It Necessary To Do Base Quality Score Recalibration Before Variants Detection In Gatk
gravatar for Lds
8.7 years ago by
Lds420 wrote:


As it mentioned in here, the reported base quality scores (the QUAL column in BAM file) before Base Quality Score Recalibration(BQSR) are neither very accurate nor informative, After recalibration, the quality scores in the QUAL field in each read in the output BAM are more accurate and then the reported quality score is closer to its actual probability of mismatching the reference genome. Is it necessary to do such step? How did the base quality affect the following variants detection?

Thanks in advance

gatk • 4.6k views
ADD COMMENTlink written 8.7 years ago by Lds420
gravatar for Zev.Kronenberg
8.7 years ago by
United States
Zev.Kronenberg11k wrote:

We do recalibration when there are multiple read groups. I believe it helps, but have no measures to back my claim. Try it both ways and see what you get. If you're trying to find disease genes eliminating any false positives really helps out.

ADD COMMENTlink written 8.7 years ago by Zev.Kronenberg11k
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