For a region:
sequence depths in control ~~sample is like: 1,2,3,4,4,4,4,4,4,4,4,4,,4,4,4,,4,4,,4,4,4,3,3,2,1
sequence depths in treatment sample is like: 1,2,3,4,5,6,7,8,9,7,7,7,7,7,8,9,8,7,6,5,4,3,2,1,1
so this is the peak , yes or no ??
the depths in treatment must be higher than control !! and the depths in treatment should be double peaks ( if draws in figures) , and the depths in control must be a horizontal line , yes or right ?
The reason I have this question is
GSM1278641 Xu_MUT_rep1_BAF155_MUT SRR1042593 GSM1278642 Xu_MUT_rep1_Input SRR1042594 848M Jun 28 14:31 SRR1042593.bam 2.7G Jun 28 14:52 SRR1042594.bam nohup time ~/.local/bin/macs2 callpeak -c SRR1042594.sam -t SRR1042593.sam -f SAM -p 0.01 -g hs -n Xu_MUT_rep1 2>Xu_MUT_rep1.masc2.log & samtools depth -r chr10:42385331-42385599 SRR1042593.sorted.bam samtools depth -r chr10:42385331-42385599 SRR1042594.sorted.bam
both samples' depths have double peaks , and the depths in control are bigger than treatment !!! ???
which really confuse me .
sorry guys, I am really not familar with discribe the question in English, hope you can get it .