Question: What's the meaning behind the Affy microarray probe ID order?
gravatar for ZHANG Li
2.1 years ago by
Hong Kong
ZHANG Li0 wrote:

Hi everyone,

I have downloaded a dataset about Affymetrix gene expression from GEO, and I found that the probes ID are listed like:









and each of them is followed by the expression value. I know that all these probe ID can be converted to gene names. But my question is what's the meaning of the probe ID order? Why they are sorted like this and what's the meaning behind the sorting? I have tried to convert all probe IDs into gene symbols but can't find a clue about why they should be sorted in this way. It's like a random arrangement but I can't find any evidence either.

Thank you.

probe id microarray • 808 views
ADD COMMENTlink modified 2.1 years ago by mastal5111.9k • written 2.1 years ago by ZHANG Li0
gravatar for mastal511
2.1 years ago by
mastal5111.9k wrote:

There is no meaning to the microarray IDs. Probesets are designed by clustering mRNAs, cDNAs and ESTs for the species of interest. Sometimes the probe IDs might have the same ID as the UniGene cluster ID that the probeset was based on.

ADD COMMENTlink written 2.1 years ago by mastal5111.9k

Thank you very much! I am quite new in this field. So...can this be understood as the measured genes are generally organized randomly?

ADD REPLYlink written 2.1 years ago by ZHANG Li0
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