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7.8 years ago
s.maxouri
•
0
Hello,
I am working with TCGA gene expression data (microarray based) level 3 for breast cancer and I have seen that the majority of the expression values is negative, even in genes that are known to be overexpressed. From what I understand the expression values are calculated as the log ratio Cy5(sample)/Cy3(universal human reference), so all the genes that are overexpressed should be above 0.
Could anyone tell me what these negative values mean? Am I missing a normalization step?
Thanks!