Question: TCGA mRNA expression data for Breast Cancer: negative expression values in overexpressed genes
0
gravatar for s.maxouri
2.9 years ago by
s.maxouri0
s.maxouri0 wrote:

Hello,

I am working with TCGA gene expression data (microarray based) level 3 for breast cancer and I have seen that the majority of the expression values is negative, even in genes that are known to be overexpressed. From what I understand the expression values are calculated as the log ratio Cy5(sample)/Cy3(universal human reference), so all the genes that are overexpressed should be above 0.

Could anyone tell me what these negative values mean? Am I missing a normalization step?

Thanks!

ADD COMMENTlink written 2.9 years ago by s.maxouri0
Please log in to add an answer.

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 1015 users visited in the last hour