Have .ab1 of 2 alleles in hybrid and .fa for 1, need .fa for the other
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5.2 years ago
michael.nagle ▴ 100

I amplified genes in E. grandis x urophylla and had them sequenced by Sanger. I have .ab1 files showing double peaks that clearly look like SNPs in the grandis and urophylla alleles. I also have the published grandis .fa for these genes.

Without scrolling through, clicking and editing for every double peak, how can I get a .fa for the urophylla allele?

alleles sanger double peak trace • 1.1k views
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