how to calculate how many orfs in transcripts
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5.3 years ago

Hi,there. Recently, I have got long, high-quality, consensus transcripts sequences, and I want to see weather there are polycistronic transcripts in my PacBia data.I have extracted the long open reading frames and predicted the likely coding regions by using TransDecoder.Then, I don't know how to calculate how many orfs in transcripts and filter for polycistronic candidates and get support from genome-based annotation. Any hints or links would be appreciated.Thank you!

pbtranscript-TOFU • 1.5k views
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